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- Title
Effects of Hovenia dulcis Thunb. extract and methyl vanillate on atopic dermatitis-like skin lesions and TNF-α/ IFN-γ-induced chemokines production in HaCaT cells.
- Authors
Lim, Sue Ji; Kim, Myungsuk; Randy, Ahmad; Nam, Eui Jeong; Nho, Chu Won
- Abstract
Objectives Here, we hypothesized that Hovenia dulcis branch extract ( HDB) and its active constituents ameliorates 2,4-dinitrochlorobenzene-induced atopic dermatitis ( AD)-like skin lesions by modulating the T helper Th1/Th2 balance in NC/Nga mice and TNF-α- and IFN-γ-induced production of thymus and activation-regulated chemokine ( TARC) and macrophage-derived chemokine ( MDC) in HaCaT cells. Methods HaCaT cells were stimulated by TNF-α/ IFN-γ in the presence of HDB and its constituents. TARC and MDC were measured by ELISA and RT- PCR. For the in-vivo study, oral feeding of HDB was performed for 5 weeks with 2,4-dinitrochlorobenzene ( DNCB) treatment every other day. The efficacy of HDB on parameters of DNCB-induced AD was evaluated morphologically, physiologically and immunologically. Key findings In-vitro studies showed that HDB and its constituents suppressed TNF-α/ IFN-γ-induced production of TARC and MDC in HaCaT cells by inhibiting MAPK signalling. In-vivo studies showed that HDB regulated immunoglobulin (Ig) E and immunoglobulin G2a (IgG2a) levels in serum and the expression of mRNA for Th1- and Th2-related mediators in skin lesions. Histopathological analyses revealed reduced epidermal thickness and reduced infiltration of skin lesions by inflammatory cells. Conclusion These results suggest that HDB inhibits AD-like skin diseases by regulating Th1 and Th2 responses in NC/Nga mice and in HaCaT cells.
- Subjects
HOVENIA dulcis; ATOPIC dermatitis; TUMOR necrosis factors; DINITROCHLOROBENZENE; INTERLEUKINS
- Publication
Journal of Pharmacy & Pharmacology, 2016, Vol 68, Issue 11, p1465
- ISSN
0022-3573
- Publication type
Article
- DOI
10.1111/jphp.12640