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- Title
T-DNA integration into the Arabidopsis genome depends on sequences of pre-insertion sites.
- Authors
Brunaud, Véronique; Balzergue Bertrand#Dubreucq, Sandrine; Aubourg, Sébastien; Samson, Franck; Chauvin, Stéphanie; Bechtold, Nicole; Cruaud, Corinne; DeRose, Richard; Pelletier, Georges; Lepiniec, Loïc; Caboche, Michel; Lecharny, Alain
- Abstract
A statistical analysis of 9000 flanking sequence tags characterizing transferred DNA (T-DNA) transformants in Arabidopsis sheds new light on T-DNA insertion by illegitimate recombination. T-DNA integration is favoured in plant DNA regions with an A-T-rich content. The formation of a short DNA duplex between the host DNA and the left end of the T-DNA sets the frame for the recombination. The sequence immediately downstream of the plant A-T-rich region is the master element for selling up the DNA duplex, and deletions into the left end of the integrated T-DNA depend on the location of a complementary sequence on the T-DNA. Recombination at the right end of the T-DNA with the host DNA involves another DNA duplex, 2-3 base pairs long, that preferentially includes a G close to the right end of the T-DNA.
- Subjects
DNA; ARABIDOPSIS; GENOMES; PLANTS; NUCLEIC acids; STATISTICS
- Publication
EMBO Reports, 2002, Vol 3, Issue 12, p1152
- ISSN
1469-221X
- Publication type
Article
- DOI
10.1093/embo-reports/kvf237