We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
In Vitro Differentiation of Mesenchymal Progenitor Cells Derived from Porcine Umbilical Cord Blood.
- Authors
Kumar, Basavarajappa Mohana; Jae-Gyu Yoo; Sun-A Ock; Jung-Gon Kim; Hye-Jin Song; Eun-Ju Kang; Seong-Keun Cho; Sung-Lim Lee; Jae-Hyeon Cho; Balasubramanian, Sivasankaran; Gyu-Jin Rho
- Abstract
Mesenchymal stem/progenitor cells (MPCs) were isolated from porcine umbilical cord blood (UCB) and their morphology, proliferation, cell cycle status, cellsurface antigen profile and expression of hematopoietic cytokines were characterized. Their capacity to differentiate in vitro into osteocytes, adipocytes and chondrocytes was also evaluated. Primary cultures of adherent porcine MPCs (pMPCs) exhibited a typical fibroblast-like morphology with significant renewal capacity and proliferative ability. Subsequent robust cell growth was indicated by the high percentage of quiescent (G0/G1) cells. The cells expressed the mesenchymal surface markers, CD29, CD49b and CD105, but not the hematopoietic markers, CD45 and CD133 and synthesized hematopoietic cytokines. Over 21 days of induction, the cells differentiated into osteocytes adipocytes and chondrocytes. The expression of lineage specific genes was gradually upregulated during osteogenesis, adipogenesis and chondrogenesis. We conclude that porcine umbilical cord blood contains a population of MPCs capable of self-renewal and of differentiating in vitro into three classical mesenchymal lineages.
- Publication
Molecules & Cells (Springer Nature), 2007, Vol 24, Issue 3, p343
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)07349-1