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- Title
"Cytomorphometric analysis of cell and nuclear diameter using light and phase contrast microscope in potentially malignant disorders".
- Authors
Patel, Ruchi M.; Praveena, Ruttala Sai; Swathi, Vegendla; Babu, Siddabattuni Naga Tilak; Hadassah, T. Navya; Kattappagari, Kiran Kumar
- Abstract
Introduction: Potentially malignant disorders (PMDS) such as leukoplakia, lichen planus, and oral sub-mucous fibrosis these disorders associated with tobacco habits either chewing or smoking. Exfoliative cytology is a simple, noninvasive diagnostic technique and it can be used in the early stage of PMDS. There are different methods for assessing cellular alterations including cytoplasm and nuclear area. The examination of the cytological smear can be done using a light microscope and phase contrast microscope. Aim: Cytomorphometric analysis of cell and nuclear diameter using light and phase contrast microscope in potentially malignant disorders. Materials and Methodology: Individuals with a history of burning sensation, restricted mouth opening, and a non-scrapable white patch were included. A buccal smear was taken and stained with hematoxylin and eosin stain and a Papainicolous stain was done. Each smear of 50 cells was selected for quantitative assessment for the nuclear and cytoplasm areas. Results: Males were more in leukoplakia, the mean age was 35.0 ± 7.1. Comparison between light and phase contrast microscope in both nuclear and cytoplasmic areas showed statistically significant. Conclusion: Oral cytomorphometric analysis will improve diagnostic reliability. This method can be one of the adjuvants it will help to detect as well as follow-up the potentially malignant disorders.
- Subjects
CELL analysis; HEMATOXYLIN &; eosin staining; EXFOLIATIVE cytology; TOBACCO use; MICROSCOPES
- Publication
JIDA: Journal of Indian Dental Association, 2023, Vol 17, Issue 3, p15
- ISSN
0019-4611
- Publication type
Article
- DOI
10.33882/jida.15.32262