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- Title
Cathepsin S Is More Abundant in Serum of Mycobacterium avium subsp. paratuberculosis -Infected Dairy Cows.
- Authors
Duda, Heidi C.; von Toerne, Christine; Korbonits, Lucia; Didier, Andrea; Scholz, Armin M.; Märtlbauer, Erwin; Hauck, Stefanie M.; Deeg, Cornelia A.
- Abstract
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of bovine paratuberculosis, a chronic granulomatous enteritis leading to economic losses and posing a risk to human health due to its zoonotic potential. The pathogen cannot reliably be detected by standard methods, and immunological procedures during the infection are not well understood. Therefore, the aim of our study was to explore host–pathogen interactions in MAP-infected dairy cows and to improve diagnostic tests. Serum proteomics analysis using quantitative label-free LC-MS/MS revealed 60 differentially abundant proteins in MAP-infected dairy cows compared to healthy controls from the same infected herd and 90 differentially abundant proteins in comparison to another control group from an uninfected herd. Pathway enrichment analysis provided new insights into the immune response to MAP and susceptibility to the infection. Furthermore, we found a higher abundance of Cathepsin S (CTSS) in the serum of MAP-infected dairy cows, which is involved in multiple enriched pathways associated with the immune system. Confirmed with Western blotting, we identified CTSS as a potential biomarker for bovine paratuberculosis. This study enabled a better understanding of procedures in the host–pathogen response to MAP and improved detection of paratuberculosis-diseased cattle.
- Subjects
MYCOBACTERIUM avium paratuberculosis; DAIRY cattle; CROHN'S disease; BLOOD serum analysis; PARATUBERCULOSIS; MASTITIS; LACTATION in cattle
- Publication
Metabolites (2218-1989), 2024, Vol 14, Issue 4, p215
- ISSN
2218-1989
- Publication type
Article
- DOI
10.3390/metabo14040215