We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Everolimus-Induced Immune Effects after Heart Transplantation: A Possible Tool for Clinicians to Monitor Patients at Risk for Transplant Rejection.
- Authors
Klaeske, Kristin; Lehmann, Sven; Palitzsch, Robert; Büttner, Petra; Barten, Markus J.; Jawad, Khalil; Eifert, Sandra; Saeed, Diyar; Borger, Michael A.; Dieterlen, Maja-Theresa
- Abstract
Background: Patients treated with an inhibitor of the mechanistic target of rapamycin (mTORI) in a calcineurin inhibitor (CNI)-free immunosuppressive regimen after heart transplantation (HTx) show a higher risk for transplant rejection. We developed an immunological monitoring tool that may improve the identification of mTORI-treated patients at risk for rejection. Methods: Circulating dendritic cells (DCs) and regulatory T cells (Tregs) were analysed in 19 mTORI- and 20 CNI-treated HTx patients by flow cytometry. Principal component and cluster analysis were used to identify patients at risk for transplant rejection. Results: The percentages of total Tregs (p = 0.02) and CD39+ Tregs (p = 0.05) were higher in mTORI-treated patients than in CNI-treated patients. The principal component analysis revealed that BDCA1+, BDCA2+ and BDCA4+ DCs as well as total Tregs could distinguish between non-rejecting and rejecting mTORI-treated patients. Most mTORI-treated rejectors showed higher levels of BDCA2+ and BDCA4+ plasmacytoid DCs and lower levels of BDCA1+ myeloid DCs and Tregs than mTORI non-rejectors. Conclusion: An mTORI-based immunosuppressive regimen induced a sufficient, tolerance-promoting reaction in Tregs, but an insufficient, adverse effect in DCs. On the basis of patient-specific immunological profiles, we established a flow cytometry-based monitoring tool that may be helpful in identifying patients at risk for rejection.
- Subjects
GRAFT rejection; HEART transplantation; REGULATORY T cells; PATIENT monitoring; HEART; PRINCIPAL components analysis
- Publication
Life (2075-1729), 2021, Vol 11, Issue 12, p1373
- ISSN
2075-1729
- Publication type
Article
- DOI
10.3390/life11121373