We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Bioassay-guided isolation and characterization of antibacterial compound from Aspergillus fumigatus HX-1 associated with Clam.
- Authors
Xu, Xiaowen; Guo, Siya; Chen, Hui; Zhang, Zongyi; Li, Xiangrong; Wang, Wenbin; Guo, Lei
- Abstract
This study aimed to identify a symbiotic fungus strain HX-1 with anti-Vibrio harveyi activity and isolate and identify the active compound. The HX-1 strain was identified as Aspergillus fumigatus according to the morphological characteristics and internal transcribed spacer (ITS) sequence analysis. The compound was isolated from the fermentation product of HX-1 strain through ethyl acetate extraction, silica gel and Sephadex LH-20 column chromatography, and semi-preparative HPLC techniques using an antibacterial-guided fractionation method. According to its physicochemical properties and spectral characteristics, the compound was identified as trypacidin having the same anti-V. harveyi activity as streptomycin sulfate, with the minimum inhibitory concentration of 31.25 µg/mL.
- Subjects
ASPERGILLUS fumigatus; VIBRIO harveyi; COLUMN chromatography; HIGH performance liquid chromatography; ETHYL acetate; ANTIBACTERIAL agents
- Publication
3 Biotech, 2021, Vol 11, Issue 4, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-021-02754-3