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- Title
Rapid detection of Salmonella enterica serovars by multiplex PCR.
- Authors
Jeyasekaran, G.; Raj, K. Thirumalai; Shakila, R. Jeya; Thangarani, A. Jemila; Sukumar, D.; Jailani, V. Abdul Kadhar
- Abstract
multiplex PCR based assay was developed for the identification of the genus Salmonella. Five sets of primers from different genomic sequences such as fimA, himA, hns, invA and hto genes were selected for the identification of serogroups of Salmonella enterica such as S. Typhi, S. ParatyphiA, S. Typhimurium, S. Enteritidis and S. Weltevreden. The selected primers amplified products with the sizes of 85, 123, 152, 275 and 496 bp, respectively, for the genus Salmonella. This assay was found to be highly sensitive, as it could detect 5 cells of Salmonella and 1,000 fg of genomic DNA. Amplification of DNA extracted from other genera viz. V. cholerae and E. coli yielded negative results. This assay provides specific and reliable results and allows for the cost-effective detection of Salmonella in one reaction tube in mixed bacterial communities.
- Subjects
SALMONELLA detection; POLYMERASE chain reaction; SALMONELLA typhi; SALMONELLA typhimurium; SALMONELLA enteritidis; DNA; MICROBIAL genomics; ESCHERICHIA coli; VIBRIO cholerae
- Publication
World Journal of Microbiology & Biotechnology, 2011, Vol 27, Issue 4, p953
- ISSN
0959-3993
- Publication type
Article
- DOI
10.1007/s11274-010-0538-9