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- Title
Association of cholesteryl ester transfer protein genotypes with paraoxonase-1 activity, lipid profile and oxidative stress in type 2 diabetes mellitus: A study in San Luis, Argentina.
- Authors
Siewert, Susana; Gonzalez, Irma Ines; Lucero, Roberto Osvaldo; Ojeda, Marta Susana
- Abstract
Aims/Introduction Diabetic dyslipidemia is common in type 2 diabetes. The Taq IB polymorphism in cholesteryl ester transfer protein ( CETP; B1 and B2 alleles; rs708272) is associated with changes in enzyme activity and lipid concentrations. The aim of the present study was to assess associations of CETP genotypes with lipoprotein profile, oxidant/anti-oxidant status and the plasma activity of paraoxonase-1 ( PON-1) in a population of diabetic patients living in San Luis, Argentina. Materials and Methods For oxidative stress status parameters, thiobarbituric acid-reactive substances ( TBARS) and nitric oxide ( NO) levels, and catalase and PON-1 activity were assessed in 40 patients with type 2 diabetes mellitus and 30 healthy participants. CETP polymorphism was analyzed by polymerase chain reaction-based methods. Results Type 2 diabetes mellitus had significantly higher concentrations of oxidative stress parameters: TBARS ( P < 0.0001) and catalase activity ( P < 0.0001). PON-1 activity and NO levels were significantly lower in diabetics ( P = 0.0002 and P = 0.0008, respectively). The CETP genotypes distribution among study groups was not significantly different. The B2 carriers of the Taq IB CETP polymorphism are associated with higher high-density lipoprotein cholesterol levels and PON-1 activity in control and type 2 diabetes mellitus patients. Linear regression analysis showed that there was a significant and positive correlation between the changes of PON-1 activity and high-density lipoprotein cholesterol levels in non-B1B1 (B2 carriers) in controls ( r = 0.83, P < 0.0001) and diabetic patients ( r = 0.39, P = 0.0003). Conclusions The results of the current study show that type 2 diabetes mellitus is characterized by intense oxidative stress, and that the alterations observed in the lipoprotein profile and PON-1 activity might be related to the higher CETP activity in diabetic patients as a consequence of insulin resistance.
- Subjects
SAN Luis (Argentina : Province); CHOLESTERYL ester transfer protein; LIPOPROTEINS; PARAOXONASE; OXIDATIVE stress; PEOPLE with diabetes; GENETIC polymorphisms; DYSLIPIDEMIA
- Publication
Journal of Diabetes Investigation, 2015, Vol 6, Issue 1, p67
- ISSN
2040-1116
- Publication type
Article
- DOI
10.1111/jdi.12256