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- Title
CHEMICAL COMPOSITION AND ANTI-INFLAMMATORY ACTIVITY OF ESSENTIAL OILS FROM RESIN OF COMMIPHORA SPECIES.
- Authors
Worku Dinku; Su Bin Park; Jin Boo Jeong; Chuleui Jung; Dekebo, Aman
- Abstract
Essential oils (EOs) were prepared by the hydro-distillation technique from the resins of four Commiphora species and analyzed by GC-MS. Major constituents of EOs were α-copaene (22.71%), β-caryophyllene (28.03%) and β-caryophyllene oxide (13.89%) for C. sphaerocarpa; α-pinene (29.1%) for C. africana; hexadecane (14.1%) for C. habessinica and d-cadinene (31.5%) for C. schimperi. We investigated the anti-inflammatory effects of EOs in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages by measuring nitric oxide (NO). The effect in mRNA or protein level after EO treatment were evaluated by RT-PCR and Western blot analysis, respectively. Among four Commiphora species, C. sphaerocarpa EO demonstrated a significant inhibition of LPS by 27.2±3.6% at 10 µg/mL and 62.3±5.2% at 20 µg/mL. C. sphaerocarpa EO inhibited LPS mediated iNOS over expression in both protein and mRNA level with dose dependent manner. It inhibited phosphorylation of ERK1/2, p38, ATF2. The enhanced anti-inflammatory activity of the EO of the plant was due to HO-1 expression by ROS dependent Nrf2 activation in RAW264.7 cells. These findings indicate C. sphaerocarpa EO inhibits the pro-inflammatory responses by inhibiting MAPK/ATF2, and triggering ROS/Nrf2/HO-1 signaling. Therefore, C. sphaerocarpa EO could have potential for useful therapeutic candidate preventing and treating inflammatory diseases.
- Subjects
ESSENTIAL oils; ANTI-inflammatory agents; WESTERN immunoblotting; SPECIES; PROTEIN expression
- Publication
Bulletin of the Chemical Society of Ethiopia, 2022, Vol 36, Issue 2, p399
- ISSN
1011-3924
- Publication type
Article
- DOI
10.4314/bcse.v36i2.13