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- Title
Metformin combined with p38 MAPK inhibitor improves cisplatin sensitivity in cisplatin-resistant ovarian cancer.
- Authors
YA XIE; ZHENG PENG; MINGXING SHI; MEI JI; HONGJUN GUO; HUIRONG SHI
- Abstract
The aim of the present study was to determine the effects of metformin, combined with a p38 mitogen-activated protein kinase (MAPK) inhibitor, on the sensitivity of cisplatin-resistant ovarian cancer to cisplatin. The expression and distribution of phosphorylated p38 MAPK (P-p38 MAPK) was confirmed in drug-resistant and primary ovarian cancer tissues by immunohistochemistry and western blotting. A bromodeoxyuridine ELISA kit was used to analyze the effects of metformin, SB203580, a p38 MAPK inhibitor, and metformin combined with SB203580, on the cell proliferation of SKOV3/DDP cisplatin-resistant ovarian cancer cells. The protein expression of P-p38 MAPK was significantly higher in cisplatin-resistant ovarian cancer, as compared with the primary ovarian cancer tissues. Metformin combined with SB203580 significantly enhanced the sensitivity of SKOV3/DDP cells to cisplatin. In conclusion, the p38 MAPK signaling pathway may be associated with cisplatin-resistant ovarian cancer. Metformin, combined with the p38 MAPK inhibitor, significantly increased the sensitivity of SKOV3/DDP cells to cisplatin treatment.
- Subjects
METFORMIN; CISPLATIN; OVARIAN cancer prevention; MITOGEN-activated protein kinases; CELL proliferation; IMMUNOHISTOCHEMISTRY; ENZYME-linked immunosorbent assay
- Publication
Molecular Medicine Reports, 2014, Vol 10, Issue 5, p2346
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2014.2490