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- Title
Use of Cellulase Obtained from Solid-State Fermentation of Orange and Passion Fruit Peels as an Enzymatic Pre-treatment Step for Anaerobic Digestion.
- Authors
da Silva, Anderson Felipe Viana; Santos, Liliana Andréa dos; de Melo, Allan Henrique Félix; Jucá, José Fernando Thomé; Santos, André Felipe de Melo Sales; Porto, Tatiana Souza
- Abstract
Cellulase production by solid-state fermentation (SSF) is a promising pre-treatment strategy for optimizing methane generation from passion fruit and orange peel. This study employed an experimental design the enzymatic process for cellulase production using orange peel and passion fruit peel as substrates. The biochemical methane potential (BMP) test was used to assess the effectiveness of the SSF pre-treatment in methane generation from waste products. The study results showed that passion fruit peel had a higher cellulase activity than orange peel. Moreover, the experimental design successfully optimized the enzymatic process, with a maximum cellulase activity of 13.91 and 14.46 U/mL for FPase e CMCase of passion fruit peel and 2.21 and 5.67 U/mL for FPase e CMCase of orange peel. BMP assays revealed that SSF pre-treatment increased methane production, with the most significant increases observed in orange peel waste with both granular and flocculent sludge (17 and 25 NmL/gVS) compared to passion fruit peel waste (13 and 14 NmL/gVS). Furthermore, configurations obtained a high percentage of methane (63 to 71%). The cone and logistic models exhibited superior performance in terms of coefficient of determination (0.891 to 0.991) and minimized residual squares (7.3 to 247.7 NmL/gVS). Overall, this study demonstrates SSF potential as an efficient pre-treatment method for cellulase production and methane generation from agro-industrial waste (passion fruit peel and orange). This sustainable, cost-effective approach not only reclaims waste products but also contributes to renewable energy production, offering significant implications for agro-industrial waste management and innovative biotechnological solutions.
- Subjects
ORANGE SA; PASSION fruit; ANAEROBIC digestion; CELLULASE; ORANGE peel; SOLID-state fermentation; ORANGES; FRUIT skins
- Publication
BioEnergy Research, 2024, Vol 17, Issue 2, p1288
- ISSN
1939-1234
- Publication type
Article
- DOI
10.1007/s12155-023-10691-7