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- Title
Molecular genetic and chemotaxonomic identification of the bacterium of the genus Ochrobactrum possessing oil-oxidizing and nitrogen-fixing activity.
- Authors
Korshunova, T.; Mukhamatdyarova, S.; Loginov, O.
- Abstract
It was shown that analysis of cellular fatty acids using gas chromatography-mass-spectrometry and application of the gene encoding β-subunit of DNA gyrase ( gyrB) as an alternative phylogenetic marker supplemented the taxonomic description of the biotechnologically valuable strain Ochrobactrum sp. IB DT-5.3/2 but were ineffective for species identification of the bacterium. However, based on the results of the matrixassisted laser desorption-ionization time of flight mass spectrometry of the protein fractions and our previous studies of the cultural-morphological and biochemical features, as well as sequencing of the 16S rRNA gene, we can conclude that the strain Ochrobactrum sp. IB DT-5.3/2 refers to the species O. intermedium.
- Subjects
GENE expression in bacteria; FATTY acid analysis; DNA topoisomerase II; MOLECULAR genetics; NITROGEN-fixing microorganisms; MASS spectrometry; BIOINDICATORS
- Publication
Biology Bulletin, 2017, Vol 44, Issue 5, p493
- ISSN
1062-3590
- Publication type
Article
- DOI
10.1134/S1062359017050090