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- Title
The effects of tissue inhibitor of metalloproteinases on β-dystroglycan in salivary adenoid cystic carcinoma.
- Authors
Hao Yanmei; Ma Jia; Si Chenchen; Xu Jia; Jing Jie
- Abstract
Objective To investigate the expression of β-dystroglycan (β-DG) and the roles of tissue inhibitor of metalloproteinases (TIMPs) on β-DG in salivary adenoid cystic carcinoma (SACC). Methods β-DG in highly lung metastatic cell line ACC-M and lowly lung metastatic one ACC-2 was tested by inimunocytochemistry with different concentrations (10, 15, 20, 25 p μmol•L-1) of TIMPs, and that without the regulation of TIMPs was served as controls. β-DG was detected in seven specimens of SACC and ten cases of normal salivary gland tissues which were considered as a comparison group by immunohistochemistry. Results There was no positive 13-DC immune-staining at the ACC-2 and ACC-M cell lines without TIMPs in the cell culture. β-DG expressed after the regulation of TIMPs. β-DG expression was localized predominantly in basement membrane of the acinus, while the negative results were distributed in the carcinoma cells and around the cancer cell nests. Conclusion β-DG is widely expressed by transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton, the fracture of this structure means that it is easy to invade and transfer, so restoration of β-DG expression by TIMPs is considered to be critical for successful treatment of SACC.
- Subjects
TISSUE inhibitors of metalloproteinases; SALIVARY gland diseases; DYSTROGLYCAN; CELL lines; IMMUNOHISTOCHEMISTRY; MEMBRANE proteins; THERAPEUTICS
- Publication
West China Journal of Stomatology, 2013, Vol 31, Issue 2, p118
- ISSN
1000-1182
- Publication type
Article
- DOI
10.7518/hxkq.2013.02.002