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- Title
白骨壤内生细菌的多样性及其抑菌活性分析.
- Authors
倪婕; 李菲; 李雨霖; 陆璐; 谢一嘉; 刘小玲; 余炼
- Abstract
[Objective] The diversity of endophytic bacteria in A vicennia marina of mangrove plants was analyzed and its antibacterial activity was detected, providing a theoretical basis for the development and utilization of mangrove plant microbial resources. [Method] The diversity of endophytic bacteria were analyzed by traditional dilution coating and 16S rRNA sequence identification, using mangrove plant A. marina as the experimental material, which was from Beilun Estuary National Nature Reserve, Guangxi Secondary metabolites in endophytic bacteria were obtained by fermentation, then the antibacterial activity of endophytic bacteria against five kinds of pathogenic bacteria was detected by Oxford cup method. [Result]61 strains of endophytic bacteria were isolated from the fruits, flowers,roots, leaves and skins, belonging to 36 species,20 genera,16 families, 12 otders,4 claases,3 phyla, and 8 potential new species among them. Actinomycetes were the dominant group of endophytic bacteria in A. marina. The number of species was fruit > flower > skin > mot and leaf. There were 10 genera of endophytic bacteria in the fruit, accounting for 50 % of total. There were 36 strains had antimicrobial activity against at least one indicator strain, and 4 strains had broad-spectrum antimicrobial activity. Among them, YA350 had the most significant antimicrobial activity against Bacillus subtilis and Penicillium expanse [ Conclusion]The species of endophytic bacteria isolated from the fruit, flower, skin, root and leaf of A. marina are diversified, and some of them have broad-spectrum antibacterial activity, which can be used as an important source for the development of new bioactive compounds.
- Subjects
ENDOPHYTIC bacteria; MANGROVE plants; NUMBERS of species; BACTERIAL diversity; METABOLITES; PATHOGENIC bacteria
- Publication
Southwest China Journal of Agricultural Sciences, 2020, Vol 33, Issue 9, p1991
- ISSN
1001-4829
- Publication type
Article
- DOI
10.16213/j.cnki.scjas.2020.9.017