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- Title
圈养不同性别林麝粪便菌群多样性研究.
- Authors
赵贵军; 竭航; 朱吉彬; 郑程莉; 曾德军; 张承露; 戚文华; 封孝兰
- Abstract
[Objective] We employed high-throughput 16S rRNA sequencing technology to investigate structure and composition of die fecal bacteria occurring between male and female forest musk deer. [Method] Ten male and ten female musk deer were selected as experimental animals, we sampled flesh feces and extracted total microbial DNA. The V3 - V4 region of 16S rRNA gene was amplified using universal primers PCR, the amplification products were sequenced on the Illumina MiSeq platform. The sequencing sequence was analyzed and counted by QIIME and other software. [Result]The results showed that there was no signifcant difference in bacterial richness and diversity among male and female forest musk deer(P >0.05). The effective sequences of male samples averaged 57 504 ± 8187, averaged 50 190 ± 6963 in female. Therefore, the bacterial richness and diversity of male was higher than female. The two groups all had the highest proportion of Firmi-cutes, Proteobacteria and Bacteroidetes, the proportion of Bacteroidetes in females was higher than males. The two groups all had the highest proportion of Ruminococcaceae, Lachnospiraceae and Unclassified_Clostridiale,males contained more Pseudomonadaceae and Enterobacteri-aceae. PCA,PLS-DA and Functional cluster heatmap analysis revealed differences between males and females, we also found male samples were better repeatability than female samples. Meanwhile, there were obvious individual differences in two groups. [Conclusion]In summary, the results indicated that there were no significant difference in composition of bacteria in the feces of male and female musk deer, but the same bacteria had different proportions in different individuals and groups. Male microbial components contained more potential pathogenic bacteria for example Pseudomona-daeeae, while the female microbes contained more beneficial bacteria Unclassified Bacteroidales. This might reflected capacity differences of resistance disease between in male and female.
- Subjects
PROTEOBACTERIA; PATHOGENIC bacteria; FEMALES; MALES; BACTERIAL diversity; INDIVIDUAL differences
- Publication
Southwest China Journal of Agricultural Sciences, 2019, Vol 32, Issue 7, p1652
- ISSN
1001-4829
- Publication type
Article
- DOI
10.16213/j.cnki.scjas.2019.7.031