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- Title
Biosynthesis of long-chain polyamines by crenarchaeal polyamine synthases from Hyperthermus butylicus and Pyrobaculum aerophilum
- Authors
Knott, Jürgen Manfred
- Abstract
Abstract: Polyamines are ubiquitously present in all organisms. In addition to the common polyamines, thermophilic archaea synthesize long-chain polyamines. In the present study polyamine synthases from Hyperthermus butylicus and Pyrobaculum aerophilum were cloned and their substrate specificity was analyzed. The polyamine synthase HbSpeE II from H. butylicus synthesized long-chain polyamines with high activity using the same mechanism that is used by a wide range of organisms to synthesize common polyamines, in which the aminopropyl residue derives from decarboxylated S-adenosylmethionine. This is the first polyamine synthase described that synthesizes a polyamine longer than a tetramine with high activity.
- Subjects
ARCHAEBACTERIA; BIOSYNTHESIS; POLYAMINES; THERMOPHILIC microorganisms; ADENOSYLMETHIONINE
- Publication
FEBS Letters, 2009, Vol 583, Issue 21, p3519
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.10.014