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- Title
Activation of translation via reduction by thioredoxin–thioredoxin reductase in Saccharomyces cerevisiae
- Authors
Jun, Kyung Ok; Song, Chin-Hee; Kim, Young-Bum; An, Jinseok; Oh, Jae Hyeun; Choi, Sang Ki
- Abstract
Abstract: Previously we reported that in vitro translation activity in extracts of Saccharomyces cerevisiae was stimulated by dithiothreitol (DTT) and further increased by the addition of thioredoxin (TRX1) [Choi, S.K. (2007) Thioredoxin-mediated regulation of protein synthesis by redox in Saccharomyces cerevisiae. Kor. J. Microbiol. Biotechnol. 35, 36–40]. To identify the pathway affecting translation, we cloned and purified thioredoxin reductase 1 (TRR1), thioredoxin reductase 2 (TRR2), glutaredoxin 1 (GRX1) and glutaredoxin reductase 1 (GLR1) as fusion proteins. Thioredoxin-mediated activation of translation was more effectively stimulated by NADPH or NADH than by DTT. Moreover, addition of TRR1 led to a further increase of translation in the presence of thioredoxin plus NADPH. These findings indicate that redox control via the thioredoxin–thioredoxin reductase system plays an important role in the regulation of translation.
- Subjects
GENETIC translation; THIOREDOXIN; SACCHAROMYCES cerevisiae; PLANT extracts; PROTEIN synthesis; CELLULAR control mechanisms; GLUTAREDOXIN; NAD(P)H dehydrogenases
- Publication
FEBS Letters, 2009, Vol 583, Issue 17, p2804
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.07.030