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- Title
Osteoactivin is a novel osteoclastic protein and plays a key role in osteoclast differentiation and activity
- Authors
Sheng, Matilda H.-C.; Wergedal, Jon E.; Mohan, Subburaman; Lau, K.-H. William
- Abstract
Abstract: This study presents gene expression, protein expression, and in situ immunohistochemical evidence that osteoclasts express high levels of osteoactivin (OA), which had previously been reported to be an osteoblast-specific protein in bone. OA expression in osteoclasts was up-regulated upon receptor activator of NFκB ligand-induced differentiation. Suppression of functional activity of OA with neutralizing antibody reduced cell size, number of nuclei, fusion, and bone resorption activity of osteoclasts. OA was co-immunoprecipitated with integrin β3 and β1, indicating that OA co-localizes with integrin β3 and/or β1 in a hetero-polymeric complex in osteoclasts. These findings indicate that OA is a novel osteoclastic protein and plays a role in osteoclast differentiation and/or activity.
- Subjects
OSTEOCLASTS; BONE cells; GENE expression; IMMUNOHISTOCHEMISTRY
- Publication
FEBS Letters, 2008, Vol 582, Issue 10, p1451
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2008.03.030