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- Title
Sp1-dependent regulation of Myeloid Elf-1 like factor in human epithelial cells
- Authors
Koga, Tomoaki; Suico, Mary Ann; Nakamura, Hideaki; Taura, Manabu; Lu, Zhuo; Shuto, Tsuyoshi; Okiyoneda, Tsukasa; Kai, Hirofumi
- Abstract
Abstract: Myeloid Elf-1 like factor (MEF) is an ETS protein, which activates the promoters of granulocyte macrophage colony-stimulating factor, interleukin-3, lysozyme, human beta defensin-2 and perforin. In spite of its many known functions, little is known about MEF transcriptional regulation. Here, we cloned the 5′-flanking region of human MEF gene and identified a TATA-less promoter region at −204/−54 which contains 4 putative binding sites for Sp1, two of which are essential in up-regulating MEF activity. These were proven by EMSA and blocking Sp1 using RNAi or mithramycin A treatment of HEK293 cells. Our results suggest that Sp1 constitutively regulates the MEF gene.
- Subjects
EPITHELIAL cells; GENETIC regulation; EVENT marketing; EXFOLIATIVE cytology
- Publication
FEBS Letters, 2005, Vol 579, Issue 13, p2811
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2005.04.015