We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Syndecan-dependent binding of Drosophila hemocytes to laminin α3/5 chain LG4-5 modules: potential role in sessile hemocyte islets formation
- Authors
Narita, Ryo; Yamashita, Hironobu; Goto, Akira; Imai, Hiromi; Ichihara, Shigeyuki; Mori, Hitoshi; Kitagawa, Yasuo
- Abstract
Heparin-column chromatography and elastase-digestion of medium from hemocyte Kc167 gave Drosophila laminin α3/5<f>βγ</f> trimer, α3/5LG2-3 and α3/5LG4-5 modules with eluting NaCl concentrations of 450, 280 and 450 mM, respectively. Kc167 cells bound dish surface with α3/5<f>βγ</f> trimer or α3/5LG4-5, but not with α3/5LG2-3 modules. Cell binding was counteracted by treating with heparin or heparan sulfate. RNA interference of syndecan in Kc167 cells impaired the binding, but that of dally or dally-like did not. Green fluorescent protein-expressing hemocytes also bound surface with α3/5<f>βγ</f> trimer or α3/5LG4-5 module. Thus, syndecan-dependent binding of hemocytes to laminin may have a potential role in sessile hemocytes islets formation in T2-A8 segments of Drosophila.
- Subjects
PROTEINS; BLOOD cells; BLOOD platelets; BIOMOLECULES
- Publication
FEBS Letters, 2004, Vol 576, Issue 1/2, p127
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2004.08.073