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- Title
Bone Marrow Dendritic Cells Internalize Live RF-81 Bovine Rotavirus and Rotavirus-like Particles (RF 2/6-GFP-VLP and RF 8*2/6/7-VLP) But are Only Activated by Live Bovine Rotavirus.
- Authors
Istrate, C.; Douagi, I.; Charpilienne, A.; McInerney, G. M.; Hidmark, Å.; Johansen, K.; Larsson, M.; Magnusson, K.-E.; Poncet, D.; Svensson, L.; Hinkula, J.
- Abstract
Dendritic cells (DC) represent the link between innate and adaptive immunity. They are classified as antigen-presenting cells (APC) and can initiate and modulate the immune response. To investigate the interaction with DCs, live RF-81 bovine rotavirus strain (RFV) and rotavirus-like particles (rota-VLP), RF 2/6-GFP-VLP and rota RF 8*2/6/7-VLP, were added in vitro to murine bone marrow-derived DCs (bmDCs). Live RFV, RF 2/6-GFP-VLP and RF 8*2/6/7-VLP all bound to bmDC and were internalized but only live RFV stimulated phenotypic maturation of the bmDCs as shown by the upregulation of the co-stimulatory molecule CD86. Even though bmDCs internalized RF 2/6-GFP-VLP and RF 8*2/6/7-VLP as efficiently as live RFV, these rota-VLP were not able to activate the cells. Supernatants derived from bmDC cultures treated with live RFV, RF 2/6-GFP-VLP or RF 8*2/6/7-VLP were examined for TNF- α production. At 6, 18 and 24 h post-infection, TNF- α concentrations were significantly increased in cultures treated with live RFV and rota-VLP compared with untreated cultures. In conclusion, this study showed that live RF-81 bovine rotavirus strain was internalized and induced bmDCs activation, whereas both RF 2/6-GFP-VLP and RF 8*2/6/7-VLP were internalized by bmDCs without triggering their activation.
- Subjects
BONE marrow cells; DENDRITIC cells; ROTAVIRUSES; ROTAVIRUS diseases; ANTIGEN presenting cells; IMMUNOCOMPETENT cells
- Publication
Scandinavian Journal of Immunology, 2007, Vol 65, Issue 6, p494
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.2007.01907.x