We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Activation of Natural Killer-Like YT-INDY Cells by Oligodeoxynucleotides and Binding by Homologous Pattern Recognition Proteins.
- Authors
Kaur, H.; Jaso-Friedmann, L.; Leary, J. H.; Praveen, K.; Brahmi, Z.; Evans, D. L.
- Abstract
The present study was designed to examine the binding and signalling effects of single base and CpG dinucleotide phosphodiester (Po) oligodeoxynucleotides (ODN) on the human natural killer (NK)-like cell line (YT-INDY). Single base Po ODN composed of 20-mers of guanosine (dG20), adenosine (dA20), cytosine (dC20) or thymidine (dT20) as well as ‘conventional’ Po CpG ODN were examined for their ability to bind and activate YT-INDY cells. Binding by dG20 and CpG ODN to YT-INDY cells was saturable and specific. dG20 binding was competitively inhibited by homologous dG20 and heterologous CpG ODN but not by dC20 and dA20. Two different YT-INDY membrane proteins (18 and 29 kDa) were identified by ligand (Southwestern) blotting with biotinylated dG20 and CpG. The specificity of the ODN-binding protein(s) was further confirmed by ODN depletion experiments using a teleost recombinant protein orthologue [nonspecific cytotoxic cells (NCC) cationic antimicrobial protein-1 (ncamp-1)] known to bind CpG and dG20. Cell proliferation and activation studies showed that dG20 and CpG treatment of YT-INDY cells induced cellular DNA synthesis (i.e. G1 to S-phase conversion). This signalling function was accompanied in dG20-treated cells by proliferation 10 h posttreatment. Both dG20 and CpG ODN binding induced a calcium flux in YT-INDY cells within seconds of treatment. These experiments demonstrated that Po single base dG20 and CpG ODN bind to a (potential) new class of cell-surface proteins that mediate the activation of YT-INDY cells.
- Subjects
NUCLEOTIDES; GUANOSINE triphosphatase; ADENOSINES; PROTEINS; CELL proliferation; HISTOLOGY
- Publication
Scandinavian Journal of Immunology, 2005, Vol 62, Issue 4, p361
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.2005.01665.x