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- Title
Penicillin-Binding Protein 7/8 Contributes to the Survival of Acinetobacter baumannii In Vitro and In Vivo.
- Authors
Russo, Thomas A.; MacDonald, Ulrike; Beanan, Janet M.; Olson, Ruth; MacDonald, Ian J.; Sauberan, Shauna L.; Luke, Nicole R.; Schultz, L. Wayne; Umland, Timothy C.
- Abstract
Background. Acinetobacter baumannii is a bacterial pathogen of increasing medical importance. Little is known about genes important for its survival in vivo. Methods and results. Screening of random transposon mutants of the model pathogen AB307-0294 identified the mutant AB307.27. AB307.27 contained its transposon insertion in pbpG, which encodes the putative lowmolecular- mass penicillin-binding protein 7/8 (PBP-7/8). AB307.27 was significantly killed in ascites (P < .001), but its growth in Luria-Bertani broth was similar to that of its parent, AB307-0294 (P = .13). The survival of AB307.27 was significantly decreased in a rat soft-tissue infection model (P < .001) and a rat pneumonia model (P = .002), compared with AB307-0294. AB307.27 was significantly killed in 90% human serum in vitro, compared with AB307- 0294 (P < .001). Electron microscopy demonstrated more coccobacillary forms of AB307.27, compared with AB307-0294, suggesting a possible modulation in the peptidoglycan, which may affect susceptibility to host defense factors. Conclusions. These findings demonstrate that PBP-7/8 contributes to the pathogenesis of A. baumannii. PBP-7/8 either directly or indirectly contributes to the resistance of AB307-0294 to complement-mediated bactericidal activity. An understanding of how PBP-7/8 contributes to serum resistance will lend insight into the role of this lowmolecular- mass PBP whose function is poorly understood.
- Subjects
CARRIER proteins; ACINETOBACTER; ACINETOBACTER infections; PEPTIDOGLYCANS; PNEUMONIA; LABORATORY rats
- Publication
Journal of Infectious Diseases, 2009, Vol 199, Issue 4, p513
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1086/596317