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- Title
Multicenter evaluation of individual donor nucleic acid testing (NAT) for simultaneous detection of human immunodeficiency virus -1 & hepatitis B & C viruses in Indian blood donors.
- Authors
Makroo, R. N.; Choudhury, N.; Jagannathan, L.; Parihar-Malhotra, M.; Raina, V.; Chaudhary, R. K.; Marwaha, N.; Bhatiad, N. K.; Gangulydd, A. K.
- Abstract
Background & objectives: India has a high prevalence of HIV-1, hapatitis C and B virus (HCV and HBV) in the blood donors but has yet to implement nucleic acid testing (NAT) in blood screening. We undertook a multicentre evaluation of blood donor testing by NAT for simultaneous detection of HIV-1, HBV and HCV in a single tube and also to determine the feasibility of NAT implementation in India's low volume setting. Methods: A total of 12,224 unlinked samples along with their serological results were obtained from representative eight blood banks in India and were individually manually tested by the Procleix® Ultrio® Assay (Chiron Corp. Emeryville, CA) for simultaneous detection of HIV-1, HCV, and HBV. Results: Of the 12,224 samples tested, 209 (1.71%) were seroreactive. One hundred thirty three samples (1.09%) were reactive by Ultrio assay, 84 samples were seroreactive but NAT non reactive. There were eight NAT yield cases: 1 HIV, 1 HIV-HCV co-infection, and 6 HBV. Interpretation & conclusions: Our observed NAT yield for all three viruses was 1 in 1528 (0.065%). We estimate NAT could interdict 3272 infectious donations a year among our approximate 5 million annual donations.
- Subjects
INDIA; BLOOD testing; HIV; HEPATITIS B virus; HEPATITIS C virus; BLOOD collection
- Publication
Indian Journal of Medical Research, 2008, Vol 127, Issue 2, p140
- ISSN
0971-5916
- Publication type
Article