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- Title
Comparative study of mesenchymal stem cells from C57BL/10 and mdx mice.
- Authors
Yong Li; Cheng Zhang; Fu Xiong; Mei-juan Yu; Fu-lin Peng; Yanchang Shang; Cui-ping Zhao; Yong-feng Xu; Zheng-shan Liu; Chang Zhou; Jin-lang Wu
- Abstract
Background: Human mesenchymal stem cells (MSCs) have been studied and applied extensively because of their ability to self-renew and differentiate into various cell types. Since most human diseases models are murine, mouse MSCs should have been studied in detail. The mdx mouse -- a Duchenne muscular dystrophy model -- was produced by introducing a point mutation in the dystrophin gene. To understand the role of dystrophin in MSCs, we compared MSCs from mdx and C57BL/10 mice, focusing particularly on the aspects of light and electron microscopic morphology, immunophenotyping, and differentiation potential. Results: Our study showed that at passage 10, mdx-MSCs exhibited increased heterochromatin, larger vacuoles, and more lysosomes under electron microscopy compared to C57BL/10-MSCs. C57BL/10-MSCs formed a few myotubes, while mdx-MSCs did not at the same passages. By passage 21, mdx-MSCs but not C57BL/10-MSCs had gradually lost their proliferative ability. In addition, a significant difference in the expression of CD34, not Sca-1 and CD11b, was observed between the MSCs from the 2 mice. Conclusion: Our current study reveals that the MSCs from the 2 mice, namely, C57BL/10 and mdx, exhibit differences in proliferative and myogenic abilities. The results suggest that the changes mouse MSC behavior may be influenced by lack of dystrophin protein in mdx mouse.
- Subjects
STEM cells; MARMOSA; CELL differentiation; DYSTROPHIN genes; MEMBRANE proteins; CYTOSKELETAL proteins; MICE
- Publication
BMC Cell Biology, 2008, Vol 9, p1
- ISSN
1471-2121
- Publication type
Article
- DOI
10.1186/1471-2121-9-24