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- Title
Design of a Novel Plasminogen Activator Based on the Structure of Hirudin.
- Authors
Yu-Gao ZHANG; Lu YUE; Yu-Xiong WANG; Xian-Mei TAO; Hou-Yan SONG
- Abstract
Using a phage library, seven peptide sequences with high affinity to human microplasminogen were obtained. Caseinolytic assay indicated that only the synthesized peptide P07 had slight fibrinolytic activity. To enhance its plasminogen activation ability, peptide P07 was fused into loop 32–35 of hirudin. In vitro assay demonstrated that this hirudin-like fusion protein can activate human plasminogen and retain the function of thrombin inhibition. Fusing the sequence “SPDASRL” into hirudin generated a plasminogen activation activity 100 times higher than peptide P07 in chromogenic and radial caseinolytic assay. This significant functional improvement might originate from a more specific active structure due to the hirudin scaffold. Edited by Chang-De LU
- Subjects
PLASMINOGEN activators; PROTEINS; FIBRINOLYTIC agents; PEPTIDES; ANTITHROMBINS; BACTERIOPHAGES; MICROBIOLOGICAL assay
- Publication
Acta Biochimica et Biophysica Sinica, 2006, Vol 38, Issue 8, p531
- ISSN
1672-9145
- Publication type
Article
- DOI
10.1111/j.1745-7270.2006.00199.x