We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
AcsS Negatively Regulates the Transcription of type VI Secretion System 2 Genes in Vibrio parahaemolyticus.
- Authors
Ni, Bin; Li, Wanpeng; Chang, Jingyang; Zhou, Yining; Li, Xue; Tian, Zhukang; Zhang, Miaomiao; Zhang, Nan; Luo, Xi; Zhang, Yiquan; Lu, Renfei
- Abstract
The type VI secretion system 2 (T6SS2) gene cluster of Vibrio parahaemolyticus comprises three operons: VPA1027-1024, VPA1043-1028, and VPA1044-1046. AcsS is a LysR-like transcriptional regulator that play a role in activating flagella-driven motility in V. parahaemolyticus. However, its potential roles in other cellular pathways remain poorly understood. In this study, we conducted a series of experiments to investigate the regulatory effects of AcsS on the transcription of VPA1027 (hcp2), VPA1043, and VPA1044. The findings revealed that AcsS indirectly inhibits the transcription of these genes. Additionally, deletion of acsS resulted in enhanced adhesion of V. parahaemolyticus to HeLa cells. However, disruption of T6SS2 alone or in conjunction with AcsS significantly diminished the adhesion capacity of V. parahaemolyticus to HeLa cells. Therefore, it is suggested that AcsS suppresses cell adhesion in V. parahaemolyticus by downregulating the transcription of T6SS2 genes.
- Publication
Current Microbiology, 2024, Vol 81, Issue 10, p1
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-024-03855-x