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- Title
Background K Channels KCNK3/9/15 Limit the Budding of Cell Membrane-derived Vesicles.
- Authors
Huang, Daniel; Chi, Naiwen; Chen, Shiou-Ching; Lee, Ting-Ying; Hsu, Kate
- Abstract
The main function of background two-pore potassium (K) channels KCNK3/9/15 is to stabilize the cell membrane potential. We previously observed that membrane potential depolarization enhances the release of HIV-1 viruses. Because membrane polarization affects the biomembrane directly, here we examined the effects of KCNK3/9/15 on the budding of nonviral vesicles. We found that depolarization by knocking down endogenous KCNK3/9/15 promoted secretion of cell-derived vesicles. We further used Vpu (an antagonist of KCNK3) as a model for the in vivo study of depolarization-stimulated secretion. Vpu is a HIV-1-encoded, ion channel-like protein (viroporin) capable of enhancing virus release and depolarizing the cell membrane potential. We found that Vpu could also promote nonviral vesicle release, perhaps through a similar mechanism that Vpu utilizes to promote viral particle release. Notably, T cells expressing Vpu alone became pathologically low in intracellular K and insensitive to extracellular K or membrane potential stimulation. In contrast, heterologous expression of KCNK3 in T cells stabilized the cell potentials by maintaining intracellular K. We thus concluded that KCNK3/9/15 expression limits membrane depolarization and depolarization-induced secretion at least in part by maintaining intracellular K.
- Subjects
CELL membranes; POTASSIUM channels; ION channels; T cells; MEMBRANE proteins
- Publication
Cell Biochemistry & Biophysics, 2011, Vol 61, Issue 3, p585
- ISSN
1085-9195
- Publication type
Article
- DOI
10.1007/s12013-011-9241-1