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- Title
A Small-Molecule FRET Reporter for the Real-Time Visualization of Cell-Surface Proteolytic Enzyme Functions.
- Authors
Mu, Jing; Liu, Fang; Rajab, Muhammad Shafiq; Shi, Meng; Li, Shuang; Goh, Chiching; Lu, Lei; Xu, Qing ‐ Hua; Liu, Bin; Ng, Lai Guan; Xing, Bengang
- Abstract
Real-time imaging of cell-surface-associated proteolytic enzymes is critical to better understand their performances in both physiological and pathological processes. However, most current approaches are limited by their complexity and poor membrane-anchoring properties. Herein, we have designed and synthesized a unique small-molecule fluorescent probe, which combines the principles of passive exogenous membrane insertion and Förster resonance energy transfer (FRET) to image cell-surface-localized furin-like convertase activities. The membrane-associated furin-like enzymatic cleavage of the peptide probe leads to an increased fluorescence intensity which was mainly localized on the plasma membrane of the furin-expressed cells. This small-molecule fluorescent probe may serve as a unique and reliable reporter for real-time visualization of endogenous cell-surfaceassociated proteolytic furin-like enzyme functions in live cells and tissues using one-photon and two-photon microscopy.
- Subjects
SMALL molecules; FLUORESCENCE resonance energy transfer; CELL membranes; PROTEOLYTIC enzymes; TISSUES; MICROSCOPY
- Publication
Angewandte Chemie, 2014, Vol 126, Issue 52, p14585
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201407182