A bovine serum albumin-bovine hemoglobin conjugate was prepared using 3-maleimidobenzoic acid N-hydroxysuccinimide ester as cross-linker. The conjugate was purified using DEAE Sepharose. It had an Mr of 127 kDa. Its P50 (half-saturated O2 pressure) value and Hill coefficient were 27 mm Hg and 2, respectively.