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- Title
Syntheses of hydroxybenzyl-α-glucosides by amyloglucosidase-catalyzed transglycosylation.
- Authors
Shin, Hwa Kyoung; Kong, Jai Yul; Lee, Jae Dong; Lee, Tae Ho
- Abstract
Glycosides were synthesized from soluble starch and hydroxybenzylalcohols (o, m and p) by the transglycosylation activity of amyloglucosidase from Rhizopus sp. in a water system. The synthesized glycosides were identified as hydroxybenzyl-α-glucosides, of which one molecule of hydroxybenzylalcohol was bound to 1-OH of glucose. The transglycosylation reaction was performed in a reaction system containing 50 mg starch, 50 mg hydroxybenzylalcohol, and 10 units of enzyme in pH 5.0 at 45 °C. The glycoside was rapidly hydrolyzed to glucose and hydroxybenzylalcohol by the α-glucosidase.
- Subjects
GLUCOSIDES; CARBOHYDRATES; GLUCOSE; ALCOHOL; GLYCOSYLATION; ENZYMES
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 4, p321
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1005699104814