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- Title
Optimization of lipase production by Aspergillus ibericus from oil cakes and its application in esterification reactions.
- Authors
Oliveira, Felisbela; Souza, Carlos E.; Peclat, Verônica R. O. L.; Salgado, José Manuel; Ribeiro, Bernardo D.; Coelho, Maria A. Z.; Venâncio, Armando; Belo, Isabel
- Abstract
Due to the actual emphasis in revalorization of agro-industrial wastes and cost reduction of enzymes production, this work aimed the optimization of lipase production from different oil cakes (OCs) produced in Brazil, by solid-state fermentation using Aspergillus ibericus MUM 03.49 and the application of the produced lipase in hydrolysis and esterification reactions. Higher lipase production was obtained using palm kernel oil cake (PKOC), yielding 127 ± 17 U/g of lipase (per mass of dry substrate). Through SSF optimization, using PKOC mixed with sesame oil cake (SOC) in a ratio of 0.45 g of PKOC per g total substrate at 57% moisture content (MC), a production of 460 ± 38 U/g of lipase was obtained after 6 days of fermentation. The obtained lipase was used in hydrolysis reactions, where it was observed higher production in short-chain triacylglycerols (TAGs) substrates. Also, it was applied in esterification reactions, where the formation of butyl decanoate using 5% (w/v) of biocatalyst was the most efficient. SSF of PKOC and SOC is a low cost competitive process to obtain A. ibericus lipase that can be used in aroma esters production, with application in the food industry.
- Subjects
OIL cake; ESTERIFICATION; AGRICULTURAL wastes; BIOCHEMICAL substrates; ENZYMES
- Publication
Food & Bioproducts Processing: Transactions of the Institution of Chemical Engineers Part C, 2017, Vol 102, p268
- ISSN
0960-3085
- Publication type
Article
- DOI
10.1016/j.fbp.2017.01.007