We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
DNA damage response in peripheral mouse blood leukocytes in vivo after variable, low-dose rate exposure.
- Authors
Wang, Qi; Pujol-Canadell, Monica; Taveras, Maria; Garty, Guy; Perrier, Jay; Bueno-Beti, Carlos; Shuryak, Igor; Brenner, David J.; Turner, Helen C.
- Abstract
Environmental contamination and ingestion of the radionuclide Cesium-137 (137Cs) is a large concern in fallout from a nuclear reactor accident or improvised nuclear device, and highlights the need to develop biological assays for low-dose rate, internal emitter radiation. To mimic low-dose rates attributable to fallout, we have developed a VAriable Dose-rate External 137Cs irradiatoR (VADER), which can provide arbitrarily varying and progressive low-dose rate irradiations in the range of 0.1–1.2 Gy/day, while circumventing the complexities of dealing with radioactively contaminated biomaterials. We investigated the kinetics of mouse peripheral leukocytes DNA damage response in vivo after variable, low-dose rate 137Cs exposure. C57BL/6 mice were placed in the VADER over 7 days with total accumulated dose up to 2.7 Gy. Peripheral blood response including the leukocyte depletion, apoptosis as well as its signal protein p53 and DNA repair biomarker γ-H2AX was measured. The results illustrated that blood leukocyte numbers had significantly dropped by day 7. P53 levels peaked at day 2 (total dose = 0.91 Gy) and then declined; whereas, γ-H2AX fluorescence intensity (MFI) and foci number generally increased with accumulated dose and peaked at day 5 (total dose = 2.08 Gy). ROC curve analysis for γ-H2AX provided a good discrimination of accumulated dose < 2 Gy and ≥ 2 Gy, highlighting the potential of γ-H2AX MFI as a biomarker for dosimetry in a protracted, environmental exposure scenario.
- Subjects
P53 protein; LEUCOCYTES; NUCLEAR reactor accidents; BIOLOGICAL assay; LEUKOCYTE count; MONONUCLEAR leukocytes; DNA damage
- Publication
Radiation & Environmental Biophysics, 2020, Vol 59, Issue 1, p89
- ISSN
0301-634X
- Publication type
Article
- DOI
10.1007/s00411-019-00825-x