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- Title
In vitro and in vivo activity of EDTA and antibacterial agents against the biofilm of mucoid Pseudomonas aeruginosa.
- Authors
Liu, Zhenqiu; Lin, Yaying; Lu, Qi; Li, Fang; Yu, Jialin; Wang, Zhengli; He, Yu; Song, Chao
- Abstract
Purpose: Refractory infection caused by bacterial biofilm is an important clinical problem. Pseudomonas aeruginosa is a common pathogen responsible for persistent and chronic biofilm infections. We aimed to explore the in vitro and in vivo activity of ethylenediamine tetraacetic acid (EDTA) in combination with antibacterial agents against mucoid P. aeruginosa biofilm. Methods: The minimal inhibitory concentration (MIC) and minimal bactericidal concentration of ciprofloxacin, gentamicin, and ampicillin alone or with EDTA against P. aeruginosa were determined in vitro. Extracellular polysaccharides (EPS) and structural parameters of the biofilm were monitored. P. aeruginosa was aerosolized and delivered into the lungs of guinea pigs, which were treated with ciprofloxacin with or without EDTA. The colony-forming units (CFUs) of P. aeruginosa were determined from the lungs. Results: EDTA reduced the MIC of ciprofloxacin and ampicillin by about 30-fold and that of gentamicin by twofold. EDTA reduced the biofilm EPS and the proportion of viable bacteria. The thickness, average diffusion distance, and textural entropy of EDTA-treated biofilm were significantly decreased. EDTA plus antibiotics reduced the colony counting from 10 to 10 CFU/mL. In vivo, EDTA plus ciprofloxacin had a significantly lower mean CFU/g of lung tissue (EDTA + ciprofloxacin 1.3 ± 0.19; EDTA 4.4 ± 0.57; ciprofloxacin 4.2 ± 0.47), and lung lesions were less severe compared with the single treatment groups. Conclusions: EDTA can destroy the biofilm structures of mucoid P. aeruginosa in vitro. Moreover, EDTA and ciprofloxacin had a significant bactericidal effect against biofilm in vivo.
- Subjects
BACTERIAL disease prevention; PSEUDOMONAS disease treatment; ANALYSIS of variance; ANIMAL experimentation; BIOFILMS; CIPROFLOXACIN; ETHYLENEDIAMINETETRAACETIC acid; GENTAMICIN; GUINEA pigs; LUNG diseases; MICROBIAL sensitivity tests; PSEUDOMONAS diseases; STEM cells; DISEASE relapse; AMPICILLIN; DATA analysis software; DESCRIPTIVE statistics; IN vitro studies; IN vivo studies; PHARMACODYNAMICS
- Publication
Infection, 2017, Vol 45, Issue 1, p23
- ISSN
0300-8126
- Publication type
Article
- DOI
10.1007/s15010-016-0905-z