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- Title
Identification of thymosins β and β in paediatric craniopharyngioma cystic fluid.
- Authors
Desiderio, Claudia; Martelli, Claudia; Rossetti, Diana; Rocco, Concezio; D'Angelo, Luca; Caldarelli, Massimo; Tamburrini, Gianpiero; Iavarone, Federica; Castagnola, Massimo; Messana, Irene; Cabras, Tiziana; Faa, Gavino
- Abstract
Background: Adamantinomatous craniopharyngioma is the third most recurrent paediatric brain tumour. Although histologically benign, it behaves aggressively as a malignant tumour due to invasion of the hypothalamus and visual pathways. Surgery is still the first and almost the only mode of treatment, although serious damage can occur as a consequence of tumour localization. The proteomic characterization of the intracystic tumoural fluid could contribute to the comprehension of the tumorigenesis processes and to the development of therapeutic targets to reduce cyst volume, allowing less invasive surgery and/or delay of the radical resection of the tumour mass and the collateral serious effects. Methods: Intracystic fluid was analysed by a LC-ESI-IT-MS top-down platform after acidification, deproteinization and chloroform liquid/liquid extraction. Findings: Thymosin β and β peptides were for the first time identified in the intracystic fluid of adamantinomatous craniopharyngioma by low- and high-resolution MS analysis coupled with LC. The two peptides showed the same distribution trend in the analysed samples. Thymosin β and β were present in 77 % of the analysed samples. These peptides were not found in the cerebrospinal fluid available for two patients. Interpretation: The presence of β-thymosins in the intracystic fluid of the tumour confirmed the secretion of these proteins in the extracellular environment. Due to their G-actin-sequestering activity and antiapoptotic and anti-inflammatory properties, these peptides could be strictly involved in both tumour progression and cyst development and growth.
- Subjects
THYMOSIN; PHARYNGITIS; AMELOBLASTOMA; BRAIN tumors; HISTOPATHOLOGY; PEDIATRIC pathology; PROTEOMICS; NEOPLASTIC cell transformation
- Publication
Child's Nervous System, 2013, Vol 29, Issue 6, p951
- ISSN
0256-7040
- Publication type
Article
- DOI
10.1007/s00381-013-2069-9