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- Title
Study of the molecular variation in pre-eclampsia placenta based on micro-Raman spectroscopy.
- Authors
Chen, Si-Jin; Zhang, Yuan; Ye, Xiang-Ping; Hu, Kun; Zhu, Mei-Fang; Huang, Yan-Yue; Zhong, Mei; Zhuang, Zheng-Fei
- Abstract
Purpose: Study of the molecular variation in pre-eclampsia placenta based on micro-Raman spectroscopy. Methods: Five pregnant women with pre-eclampsia from Nanfang hospital were selected as study group whose average age is 28.5 years and 38 ± 2 weeks gestation. The same period of healthy pregnant women, whose average age is 27.6 years and pregnant 39 ± 1 weeks, as control group ( n = 5). The normal and pre-eclamptic placental tissues are detected by micro-Raman spectroscopy with the spectrum resolution of 1 cm. Results: We find that the protein structure of α-helix, β-pleated sheet and β-turn is overlying in pre-eclamptic placenta, which lead to a disorder of protein structure. The Raman peaks assigned to tryptophan indole ring and phenylalanine in pre-eclamptic placental tissue are more higher than that in normal tissue. Conclusions: Results suggest that the ordered structures of the main chain in protein molecules are reduced significantly, and the amino acid of side chains is damaged obviously. And a principal component analysis is used to classify the Raman spectra between normal and pre-eclamptic placental tissues. This study presents that Raman spectroscopy has a great potential on the mechanism research and diagnosis of placental lesions.
- Subjects
PREECLAMPSIA; PLACENTA; RAMAN spectroscopy; MOLECULAR spectroscopy; HYPERTENSION in pregnancy; PREGNANCY complications; MATERNAL health
- Publication
Archives of Gynecology & Obstetrics, 2014, Vol 290, Issue 5, p943
- ISSN
0932-0067
- Publication type
Article
- DOI
10.1007/s00404-014-3282-9