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- Title
Deletion analysis of the sapovirus VP1 gene for the assembly of virus-like particles.
- Authors
Hansman, G. S.; Matsubara, N.; Oka, T.; Ogawa, S.; Natori, K.; Takeda, N.; Katayama, K.
- Abstract
Human sapovirus (SaV) strains are agents of gastroenteritis. They cannot be grown in cell culture. In this study, constructs containing SaV N- and C-terminal-deleted recombinant capsid proteins (rVP1) were expressed in a baculovirus expression system to allow us to better understand the sequence requirements for the formation of virus-like particles (VLPs). Only proteins derived from N-terminal-deleted rVP1 constructs that began 49 nucleotides downstream assembled into VLPs, which included both small and native-size VLPs. Our results were similar to those reported in a rabbit hemorrhagic disease virus (RHDV) N- and C-terminal-deleted rVP1 expression study but were distinct from those reported in a norovirus N- and C-terminal-deleted rVP1 expression study, suggesting that SaV and RHDV may have similar expression requirements.
- Subjects
VIRAL proteins; BACULOVIRUSES; NUCLEOTIDES; HEMORRHAGIC diseases; MICROBIAL proteins
- Publication
Archives of Virology, 2005, Vol 150, Issue 12, p2529
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-005-0599-5