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- Title
Polymorphism of HIV-1 gag (p17) gene from female sex workers in Calcutta, India.
- Authors
Sengupta, S.; Khetawat, D.; Jana, S.; Sarkar, K.; Bhattacharya, S. K.; Chakrabarti, S.
- Abstract
HIV-1 subtype C is the major subtype in India as evidenced from the analysis of specific regions within envelope and gag gene. The matrix protein (p17) of HIV-1 which is involved in several functions like the viral RNA transport, nuclear localization, assembly of pre-integration complex into host nucleus has been used to study the strain diversity among female sexworkers in Calcutta. The gene encoding for the HIV-1 matrix protein, p17 was amplified by nested polymerase chain reaction (PCR) from blood samples of HIV-1 seropositive female sex workers (FSW) in Calcutta, India. Genes of twenty-two samples were sequenced and the phylogenetic analysis with different global strains showed that the majority (seventeen) was clustered with Indian type C. A few samples were found to be close to other C subtypes isolated from South Africa, China and Myanmar. The comparison of Calcutta samples with the samples from other regions of India along with other non-asian subtype C sequences clearly revealed a different cluster of Indian sequences. The two samples, cal 242 and cal 709 was found to be the most divergent type and showed close relatedness with African C subtypes.
- Subjects
KOLKATA (India); INDIA; GENETIC polymorphisms; HIV infections; HIV; SEX workers
- Publication
Archives of Virology, 2005, Vol 150, Issue 10, p2117
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-005-0562-5