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- Title
Inactivation of β-lactamases from Enterobacter cloacae by monophosphams.
- Authors
Bush, Karen; Smith, S. A.; Tanaka, S. K.; Bonner, D. P.
- Abstract
Amongst the monocyclic β-lactam antibiotics, selected monophosphams were potent mechanism-based inactivators of the P99 and E2 cephalosporinases of . Inhibition of these enzymes was time-dependent with second order rate constants for inactivation of 100,000 to 20,000,000 1/mole/min. After incubation for 24 h at least 99% of the enzymatic activity was inhibited when enzyme was exposed to a ten-fold excess of inactivator. Amongst the monophosphams three classes of inhibitors were seen: irreversible inactivators as described above, transient inactivators and competitive (inhibitory) substrates.
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 1988, Vol 22, Issue 6, p801
- ISSN
0305-7453
- Publication type
Article