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- Title
Monitoring fungal viability and development in plants infected with Colletotrichum higginsianum by quantitative reverse transcription-polymerase chain reaction.
- Authors
Narusaka, Mari; Shiraishi, Tomonori; Iwabuchi, Masaki; Narusaka, Yoshihiro
- Abstract
Colletotrichum higginsianum causes typical anthracnose lesions on a wide range of cruciferous plants. Currently, assays for C. higginsianum infection of host plants rely on visual diagnosis of symptoms. In this study, we developed an assay to estimate the viability and development of C. higginsianum in Arabidopsis thaliana and Brassica rapa by detecting C. higginsianum-actin ( Ch-ACT) mRNA using quantitative reverse transcription-PCR. Ch-ACT mRNA was specifically targeted because of its constitutive expression throughout the cell cycle. The methodology described will be useful for assessing potential antifungal compounds and breeding disease-resistant crops.
- Subjects
COLLETOTRICHUM; REVERSE transcriptase polymerase chain reaction; ANTHRACNOSE; ARABIDOPSIS thaliana; CHINESE cabbage; PLANT diseases
- Publication
Journal of General Plant Pathology, 2010, Vol 76, Issue 1, p1
- ISSN
1345-2630
- Publication type
Article
- DOI
10.1007/s10327-009-0211-z