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- Title
油酸对灵芝液态深层发酵合成羊毛甾醇代谢流 分布的影响.
- Authors
冯杰; 刘艳芳; 颜梦秋; 唐传红; 周帅; 张劲松
- Abstract
The effects of oleic acid on the metabolic flux distribution of lanosterol, the final products of liquid submerged fermentation of Ganoderma lingzhi, were studied by metabolic flux method in a 3 L stirred fermentor. The results showed that during the submerged fermentation of G. lingzhi, the metabolic flux of lanosterol increased by 45.29% by adding oleic acid to the liquid substrate. The content of by-product ergosterol decreased from 3.84 mg/g to 0.02 mg/g. The metabolic fluxes of lanosterol synthesis pathway (LP), tricarboxylic acid cycle (TCA), pentose phosphate pathway (PP) and glycolysis pathway (EMP) were increased by 29.09%, 14.52%, 12.22% and 2.11% as compared with those of the controls, respectively. Therefore, the addition of oleic acid could strengthen the overall metabolic flow distribution during submerged fermentation of G. lingzhi, reduce the accumulation of ergosterol, and improve the metabolic flow of lanosterol. This study laid a foundation for the improvement of triterpenoid yield in submerged fermentation of G. lingzhi.
- Subjects
PENTOSE phosphate pathway; KREBS cycle; METABOLIC flux analysis; OLEIC acid; ERGOSTEROL
- Publication
Mycosystema, 2023, Vol 46, Issue 6, p1386
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.220248