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- Title
Validation of GCN5L1/BLOC1S1/BLOS1 antibodies using knockout cells and tissue.
- Authors
Bugga, Paramesha; Stoner, Michael W.; Manning, Janet R.; Mushala, Bellina A. S.; Bhattarai, Nisha; Sharifi-Sanjani, Maryam; Webster, Bradley R.; Thapa, Dharendra; Scott, Iain
- Abstract
GCN5L1, also known as BLOC1S1 and BLOS1, is a small intracellular protein involved in many key biological processes. Over the last decade, GCN5L1 has been implicated in the regulation of protein lysine acetylation, energy metabolism, endo-lysosomal function, and cellular immune pathways. An increasing number of published papers have used commercially-available reagents to interrogate GCN5L1 function. However, in many cases these reagents have not been rigorously validated, leading to potentially misleading results. In this report we tested several commercially-available antibodies for GCN5L1, and found that two-thirds of those available did not unambiguously detect the protein by western blot in cultured mouse cells or ex vivo liver tissue. These data suggest that previously published studies which used these unverified antibodies to measure GCN5L1 protein abundance, in the absence of other independent methods of corroboration, should be interpreted with appropriate caution.
- Subjects
WESTERN immunoblotting; ENERGY metabolism; TISSUES; PROTEIN microarrays; ACETYLATION; LYSINE
- Publication
Biochemical Journal, 2024, Vol 481, Issue 10, p643
- ISSN
0264-6021
- Publication type
Article
- DOI
10.1042/BCJ20230302