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- Title
非酒精性脂肪肝病大鼠肠道菌群对肠促胰素效应的影响.
- Authors
王亚涛; 程妍; 徐静远; 史海涛; 王凯; 鲁晓岚
- Abstract
Objective To explore the influence of gut microbiota in non-alcoholic fatty liver (NAFLD)rat on L cells,glucagon-like peptide-1(GLP-1)and its receptors(GLP-1R). Methods A total of 20 SD rats were randomly divided into 2 groups(n=10 for each group),one group was fed with normal diet(ND),the other was fed with high-fat diet(HFD).Another 20 SD rats orally administered nonabsorbed antibiotics for 2 weeks to establish a pseudo germ-free rat model,and then were divided into 2 groups (n=10),one group was colonised with the gut microbiota from HFD group,the other were colonised from ND group,and both of them were then fed with high-fat diet for an additional 8 weeks after colonisation. At the end of 8th week,the body weight was measured,the serum level of blood lipids,fasting blood glucose and insulin were assayed,the insulin resistance index(HOMA-IR)was calculated,the liver histology was evaluated,the number of colonic L cells were counted,the levels of GLP-1 in 1-hour postprandial of portal blood and colonic homogenates were determined,and the levels of GLP-1R in hepatic and pancreatic homogenates were also determined. Moreover, the amount of Bifidobacterium, Lactobacillus,Escherichia coli and Enterococcus in the faecal microbiota were detected. Results Compared with rats colonised with gut microbiota from ND group,rats colonised from HFD group showed more serious hepatic fat deposition and insulin resistance(P<0.05). They had increased body weight(P< 0.01) and increased blood lipids(P<0.05). They had decreased number of colonic L cells(P<0.05), decreased synthesis and secretion level of GLP-1(P<0.001),and decreased expression of GLP-1R in liver and pancrease(P<0.001). They had a decrease in Bifidobacterium and Lactobacillus(P<0.05),and an increase in Escherichia coli and Enterococcus in the feces(P<0.05). Conclusion Under HFD the gut microbiota of NAFLD can reduce the number of colonic L cells in rats.
- Publication
Fudan University Journal of Medical Sciences, 2022, Vol 49, Issue 3, p332
- ISSN
1672-8467
- Publication type
Article
- DOI
10.3969/j.issn.1672-8467.2022.03.003