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- Title
PCR-RFLP assay for genotyping of three different regions of the BMP15 gene in sheep from the Bulgarian dairy synthetic population.
- Authors
Bozhilova-Sakova, Milena; Dimitrova, Ivona; Stancheva, Nevyana; Nenova, Radena; Tzonev, Todor; Ignatova, Maya
- Abstract
Bone morphogenetic protein 15 (BMP15/FecX) gene is one of the candidate genes for the reproduction in farm animals, especially sheep. The present study aimed to detect the genetic polymorphism in three different regions of BMP15 gene in sheep from the Bulgarian Dairy Synthetic Population (BDSP) using PCR-RFLP technique. For the purpose of the experiment were used 163 ewes from the herd of the Agricultural Institute-Shumen with records of the number of lambs born from a minimum of two consecutive lambing. The average number lambing of ewe is 4.07. DNA was extracted from blood samples of all ewes, subjected to PCR amplification. The PCR products of 141 bp (FecXG), 153 bp (FecXB), and 240 bp (FecXH) were cut with HinfI, DdeI, and SpeI restriction enzymes, respectively. In FecXG region were visualized two fragments with length 112 bp and 29 bp (wild genotype ++) in all tested ewes. For FecXB in all animals were obtained two fragments of 122 bp and 31 bp which corresponded to homozygous wild genotype AA. For FecXH all animals were homozygous with CC genotype and only one determined fragment of 240 bp. In conclusion, the findings in present study did not detect any polymorphisms in BMP-15 gene in sheep, regardless their good prolificacy.
- Subjects
RESTRICTION fragment length polymorphisms; SHEEP; BONE morphogenetic proteins; AGRICULTURE; DOMESTIC animals; GENETIC polymorphisms; SHEEP farming
- Publication
Bulgarian Journal of Animal Husbandry / Životnov Dni Nauki, 2023, Vol 60, Issue 2, p29
- ISSN
0514-7441
- Publication type
Article