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- Title
Evaluation of Cytotoxicity and Apoptosis Induced by Coumarin Hydrazide-Hydrazone Derivatives in Human Hepatocellular Carcinoma Cell Line.
- Authors
Saisuree Prateeptongkum; Nongnaphat Duangdee; Wiratchanee Mahavorasirikul
- Abstract
Coumarin and aryl hydrazide-hydrazone have attracted our attention due to their vast biological properties. Previous studies suggested that coumarin-tethered aryl hydrazide-hydrazone showed potent activities against HepG2. In the present study, we investigated the cytotoxic potency of the coumarin derivatives 1 - 3 to compare with coumarin hydrazine-hydrazone hybrids 4 and 5 against hepatocellular carcinoma HepG2 and LH86 cell lines. Among the tested coumarins, hybrids 4 and 5 showed highly potent activity against HepG2 with IC50 values of 17.82 ± 2.79 and 7.87 ± 0.88 μg/mL, respectively. The hybrid 4 also showed the strongest activity against LH86 cell line with IC50 values of 48.32 ± 2.64 μg/mL. Further, we have studied the mechanism of action of the hybrid compounds 4 and 5 in HepG2 cells via the flowcytometry analysis and the activation of the caspase-3 and caspase-7. The results showed that hybrids 4 and 5 obviously inhibited the proliferation of HepG2 cell line through inducing apoptosis.
- Subjects
CYTOTOXINS; COUMARIN derivatives; CELL lines; HEPATOCELLULAR carcinoma; APOPTOSIS; HYDRAZINE
- Publication
Trends in Sciences, 2024, Vol 21, Issue 7, p1
- ISSN
2774-0226
- Publication type
Article
- DOI
10.48048/tis.2024.7628