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- Title
牛赤羽病胶体金抗体检测试纸条的研制.
- Authors
孔玉方; 陈冬杰; 王慧煜; 景宏丽; 许晓琳; 魏 方; 吴绍强
- Abstract
The study was to establish a colloidal gold immunochromatography test strip for rapidly detecting antibodies angainst akabane virus in bovine. the recombinant protein N was used for colloidal gold marker, and the rabbit anti-cow IgG and the rabbit anti-N protein polyclonal antibody, which sprayed on nitrocellulose membrane as test lines (T) and quality control lines (C), respectively. The result showed that when the serum dilution of bovine Akabane virus positive antibody was 1∶512, the detection line was still colored. There was no cross-reaction with positive sera of bovine viral diarrhea virus, bovine tuberculosis, brucellosis, bovine pasteurellosis, endemic bovine leukemia virus and bluetongue virus. The comparation of the established method with ELISA kit antibody detection kit for detecting 40 clinical sera indicated that the coincidence was 92.6%. The study indicates that the test strip is more suitable for grass-roots farmers to screen cattle akabane disease.
- Subjects
BOVINE leukemia virus; COLLOIDAL gold; TUBERCULOSIS in cattle; RECOMBINANT proteins; BOVINE viral diarrhea virus; VIRAL antibodies; CATTLE herding
- Publication
Feed Research, 2023, Vol 46, Issue 4, p123
- ISSN
1002-2813
- Publication type
Article
- DOI
10.13557/j.cnki.issn1002-2813.2023.04.025