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- Title
Ablation of CD22 in ligand-deficient mice restores B cell receptor signaling.
- Authors
Collins, Brian E.; Smith, Brian A.; Bengtson, Per; Paulson, James C.
- Abstract
CD22 is a negative regulator of B cell signaling, an activity modulated by its interaction with glycan ligands containing α2-6-linked sialic acids. B cells deficient in the enzyme (ST6Gal I) that forms the CD22 ligand show suppressed BCR signaling. Here we report that mice deficient in both CD22 and its ligand (Cd22−/−St6gal1−/− mice) showed restored B cell receptor (BCR) signaling, suggesting that the suppressed signaling of St6gal1−/− cells is mediated through CD22. Coincident with suppressed BCR signaling, B cells lacking ST6Gal I showed a net redistribution of the BCR to clathrin-rich microdomains containing most of the CD22, resulting in a twofold increase in the localization of CD22 together with the BCR. These studies suggest an important function for the CD22-ligand interaction in regulating BCR signaling and microdomain localization.
- Subjects
B cells; CELL receptors; LYMPHOCYTES; BINDING sites; LIGANDS (Biochemistry); CELL communication; CELL membranes
- Publication
Nature Immunology, 2006, Vol 7, Issue 2, p199
- ISSN
1529-2908
- Publication type
Article
- DOI
10.1038/ni1283