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- Title
ESTUDIO RETROSPECTIVO PARA DETERMINAR LA PRESENCIA DE Escherichia coli PRODUCTORES DE b-LACTAMASAS DE ESPECTRO EXTENDIDO Y RESISTENTES A COLISTINA EN ANIMALES PARA CONSUMO HUMANO Y EN MASCOTAS DE LA PROVINCIA DE BUENOS AIRES, ARGENTINA.
- Authors
Vinocur, F.; Nievas, V. F.; Garassino, B. J.; Rodríguez Ramos, S.; Giacoboni, G. I.; Moredo, F. A.
- Abstract
The objective of the retrospectiv:e study was to determine the presence of Eschericia coli producing extended spectrum β-lactamases and E. coli resistant to colistin, in collection isolates, obtained from fecal samples of 171 bovines, 42 broilers, 69 laying hens and 52 dogs and cats, between 2004 and 2016. As screening, they were spread on MacConkey with 4 μg/ml of cefotaxime and on MacConkey with 2 μg/ml of colistin. Susceptibility against amoxicillin/ac. clavulanate, cefotaxime, ceftazidime, cefoxitin, imipenem, and ertapenem with the CLSI diffusion method; and colistin with spot agar. Of the E. coli of avian origin, 44 grew with cefotaxime and 4 with colistin; 7 were isolated from laying hens in 2008 and 37 from broiler chickens in 2012. All were susceptible to cefoxitin, ertapenem and imipenem. 17/44 presented ESBL phenotype. blaCTX-M gene was detected in 28/44. E. coli that grew on colistin did not grow on spot agar and did not carry mcr-1. Bovine and pet origin E. coli were susceptible to all the β-lactams analyzed and to colistin. Future studies will be necessary to complete the phenotypic and genotypic characterization of the isolates and determine their behavior against other antimicrobials used in veterinary medicine and classified by the WHO as a Highest Priority due to their importance in public health.
- Publication
Veterinaria Cuyana, 2021, Vol 15, p15
- ISSN
1850-0900
- Publication type
Article