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- Title
缺血预处理通过激活自噬保护肾缺血- 再灌注损伤.
- Authors
陈钊; 田李芳; 马晓桃; 马芳霞; 姚恬; 王莉
- Abstract
Objective: To investigate the protective effect and possible mechanism of Ischemic preconditioning on acute kidney injury induced by ischemia-reperfusion. Methods: 18 healthy male Sprague-Dawley rats were randomly divided into three groups: sham operation group (Sham group), renal ischemia group (I/R group), and experimental group. The rats in the Sham group were freed from the left renal pedicle after laparotomy, without clipping, and the abdomen was observed for 60 min. In the I/R group, the right kidney was excised after laparotomy, and the left renal pedicle was separated. After 15 minutes, the left renal pedicle was continuously clamped with a non-injured artery clip for 45 min, then the abdomen was closed and the left renal blood flow was restored. In the experimental group, the right kidney was removed after laparotomy, and the left renal pedicle was separated. After 4 cycles of clamping the left renal pedicle for 1 min/reperfusion for 4 min, the injured artery was continuously clamped for 45 min, and the abdomen was closed and restored Left renal blood flow. The levels of BUN and SCR, renal histopathology score and expression of LC3 and Beclin-1 were compared between the groups. Results: All rats did not die during the course of the experiment The BUN and SCr values in the I/R group and the experimental group were significantly higher than those in the Sham group at 4 h and 24 h after reperfusion (P<0.05). The histopathological scores of the kidneys were significantly higher than those in the Sham group (P<0.05). The indicators were significantly lower than the I/R group (P<0.05). The ratio of LC3-II/LC3-I and the relative expression of Beclin-1 were significantly higher than Sham group (P<0.05) The above indexes in the experimental group were significantly lower than those in the I/R group (P<0.05). At 24 h after reperfusion, the LC3-II/LC3-I ratio and the relative expression of Beclin-1 in the experimental group were significantly correlated with renal histopathological scores, BUN and SCr values (P<0.05). Conclusions: Ischemic preconditioning may attenuate acute renal injury and induced by ischemia-reperfusion and improve renal function by activating autophagy.
- Subjects
ISCHEMIC preconditioning; ACUTE kidney failure; BLOOD flow; EXPERIMENTAL groups; FREE groups; REPERFUSION
- Publication
Progress in Modern Biomedicine, 2019, Vol 19, Issue 23, p4421
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2019.23.005